FEATURE ARTICLE
Tissue Levels of Leukemia Inhibitory Factor Vary by Osteoarthritis Grade
Abstract
El
objetivo de este estudio era observar la expresión del factor inhibidor
de la leucemia ( LIF ) en los animales y en diferentes grados clínicos
de los tejidos osteoartríticos paciente . Treinta y cinco conejos se utilizaron en un modelo experimental de Colombo de la osteoartritis ( OA ) . Cinco
conejos cada uno se sacrificaron en los días postoperatorios 3 , 7 , 14
, 28 , 42 , 56 , y 84 . Análisis de inmunohistoquímica para la
expresión de LIF y la distribución en el cartílago y la membrana
sinovial de los animales se realizó a estos tiempos . Sesenta
y siete muestras de tejido articular humano se obtuvieron de pacientes
con diferentes grados de OA de acuerdo con los síntomas y la inspección
radiográfica . La
expresión de mRNA de LIF se determinó por la transcripción de reacción
en cadena de polimerasa inversa , y la proteína de LIF se determinó
mediante ensayo de inmunoabsorción ligado a enzimas ( ELISA ) . Los resultados mostraron una ligera expresión de LIF en el tejido normal del cartílago , pero menos en el tejido sinovial ; Sin
embargo , la expresión de LIF fue marcado en las células de
revestimiento sinovial y el cartílago superficial y de capa media en la
OA de animales ( P < 0,05 ) . Leucemia
ARNm del factor inhibitorio fue expresada al más alto nivel en el hueso
subcondral moderado de degradación , y LIF fue expresada al más alto
nivel en la degradación serio tejido de cartílago articular . Estos resultados fueron similares a los encontrados con ELISA . Este estudio sugiere que la LIF en los tejidos articulares OA varía según los síntomas clínicos y de grado. Desempeña un papel importante en la patogénesis de la OA
The objective of this study was to observe the expression of leukemia inhibitory factor (LIF) in animals and in different clinical grades of patient osteoarthritic tissues. Thirty-five rabbits were used in a Colombo model of experimental osteoarthritis (OA). Five rabbits each were sacrificed on postoperative days 3, 7, 14, 28, 42, 56, and 84. Immunohistochemistry analysis for LIF expression and distribution in the cartilage and synovium of animals was performed at these times. Sixty-seven samples of human articular tissue were obtained from patients with different grades of OA according to symptoms and radiographic inspection. The mRNA expression of LIF was determined by reverse transcription polymerase chain reaction, and LIF protein was determined by enzyme-linked immunosorbent assay (ELISA). The results showed a slight expression of LIF in normal cartilage tissue but less in synovium tissue; however, the expression of LIF was marked in synovial lining cells and superficial and middle-layer cartilage in animal OA (P<.05). Leukemia inhibitory factor mRNA was expressed at the highest level in moderate degrading subchondral bone, and LIF was expressed at the highest level in seriously degrading articular cartilage tissue. These results were similar to those found with ELISA. This study suggests that LIF in OA articular tissues varies by clinical symptoms and grade. It plays an important role in the pathogenesis of OA.
The authors are from the Department of Orthopaedic Surgery (YJ), The First People’s Hospital of Shunde, Shunde Guangdong; the Department of Orthopaedic Surgery (QX), Jiangxi Province People’s Hospital, NanChang, Jiangxi; the Department of Orthopaedic Surgery (ZH, JH), The First Affiliated Hospital of Chongqing Medical University, Chongqing; and the Department of Orthopaedic Surgery (BP, JS,QY, HL), The Second Affiliated Hospital of Kunming Medical College, Kunming, China.
Drs Jiang and Xiao contributed equally to this work and should be considered as equal first authors.
The authors have no relevant financial relationships to disclose.
The authors thank Dr BaoHua Zhang for help performing the clinical statistical analysis.
Correspondence should be addressed to: ZhenMing Hu, MD, PhD, Department of Orthopaedic Surgery, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Rd, Chongqing 400016, China ( zhenminghu62@aliyun.com).
Received: September 24, 2012
Accepted: November 08, 2013
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